Recombinant Collagen Anti-wrinkle Repair Mask

Key Ingredients

recombinant human type III collagen, odium hyaluronate, trehalose, Aqua, butylene glycol, pentylene glyco, 1,2-hexanediol, hydroxyace tophenone, arginine, carbomer, allantoin, hydroxyethylcellulose, disodium phosphate, polysorbate-60, sodium phosphate

Suitable for

Ages 25+ (Early signs of aging); Fine lines and dryness lines; Facial hollows; Target audience for early anti-aging care

Indications

• Fine lines and wrinkles
• The appearance of future lines
• All skin types, such as sensitive skin.

Instructions

Cleanse and dry face thoroughly.

Remove mask from packaging, and apply to fit the contours of your face. Leave on for 20 minutes and then remove gently.

Effects

• Instant Hydration (15-20 Minutes)
• Reduce Fine Lines and Wrinkles (1-2 Weeks)
• Improve Skin Elasticity (3-4 Weeks)
• Brighten Dull Skin (2-3 Weeks)
• Tighten Pores (1-2 Weeks)
• Soothe and Calm Skin (15-20 Minutes)

Product Efficacy Report

Specific Claim (Applicable to Sensitive Skin)

-Test substance: Extruded product

-Negative control: Blank

-Lactic Acid Stinging Test:

1. Apply 50 µL of a 10% lactic acid solution onto a single-layer non-woven fabric patch (0.8 cm in diameter). Affix this patch to the nasolabial fold on one side of the subject’s face, using distilled water as a control on the opposite side.
2. Researchers ask the subjects about stinging sensations at 2.5 minutes and 5 minutes, scoring the responses according to the “4-point scale” in Table 1. Subjects with a cumulative stinging score on the lactic acid side that exceeds the score on the distilled water side by more than 3 points are classified as “lactic acid stingers” and may be determined to have sensitive skin.
3. Use qualified patch test chambers with an area not exceeding 50 mm² and a depth of approximately 1 mm. Load the test substance into the chamber; the quantity used should be approximately 0.020 g–0.025 g (for solids or semi-solids) or 0.020 mL–0.025 mL (for liquids).
4. Affix the chambers containing the test substance and the negative control to the flexor surface of the subject’s forearm; remove the chambers after 24 hours.
5. Observe skin reactions at 30 minutes (after the indentation mark has faded), 24 hours, and 48 hours following the removal of the chambers, using the criteria in Table 2, and record the observations.

-Test Results: A total of 32 subjects with sensitive skin were enrolled in this study; 31 subjects completed the trial (Subject No. 02 withdrew for personal reasons). Systematic sampling was used to select the first 30 valid subjects (excluding Subject No. 32), resulting in a final dataset of 30 participants. The group comprised 7 males and 23 females, aged 19 to 58.

-The results showed that all 30 subjects with sensitive skin received a score of 0 at 0.5, 24, and 48 hours after the removal of the patch test device, indicating no adverse skin reactions.

Anti-wrinkle and Firming

This test employs a biochemical method—specifically, a collagenase inhibition assay model—to measure the sample’s ability to inhibit collagenase activity, thereby evaluating the anti-wrinkle and firming efficacy of cosmetic raw materials and finished products.

Test Method: Samples were diluted with purified water at a specific ratio, and required components were added. The setup included: Well 1 (Test Group: sample group and positive control group), Well 2 (Test Control Blank Group), Well 3 (Negative Control Group), and Well 4 (Negative Control Blank Group).

Upon completion of the reaction, the reaction mixtures were transferred to the corresponding 96-well plates (with three rows per group), and absorbance (OD value) was measured at 335 nm using a microplate reader.

Soothing

This test evaluates the anti-allergic activity of the sample based on the hyaluronidase inhibition rate; a higher inhibition rate indicates stronger anti-allergic activity.

This test evaluates the soothing efficacy of cosmetics and raw materials by measuring the sample’s ability to inhibit hyaluronidase activity. Test Method: Components were added to the sample group (diluted with purified water to a specific ratio) and the positive control group.

Test Conclusions:

1. Under the test conditions, the collagenase inhibition rate of the sample “BONNEHEURE Recombinant Collagen Anti-Wrinkle Repair Mask” (10% concentration) was 69.5% ± 2.3% (P < 0.05, indicating a significant difference). The sample demonstrated inhibitory activity against collagenase, suggesting that the “BONNEHEURE Recombinant Collagen Anti-Wrinkle Repair Mask” possesses anti-wrinkle and firming properties.
2. Under the test conditions, the hyaluronidase inhibition rate of the sample “BONNEHEURE Recombinant Collagen Anti-Wrinkle Repair Mask” (undiluted concentration) was 70.7% ± 3.0% (P < 0.05, indicating a significant difference). The sample demonstrated inhibitory activity against hyaluronidase, suggesting that the “BONNEHEURE Recombinant Collagen Anti-Wrinkle Repair Mask” possesses soothing properties.

Moisturization

This test establishes an *in vitro* protocol to evaluate the moisturizing efficacy of cosmetics and raw materials by measuring their water-retention performance in a dry environment.

Test Method: Three replicates were set up for each test group. Negative and positive control groups were included simultaneously; the positive control used glycerol, and the negative control used purified water. The procedure for controls was identical to that for the test samples: A clean, dry, glass weighing bottle with a stopper was placed in a desiccator (containing indicating silica gel desiccant) at 25°C ± 1°C for 24 hours prior to the test, and its weight (m0) was accurately recorded. At room temperature, 1 mL of nutrient agar was pipetted into the weighing bottle; after solidification, 200 μL of the test sample was added, and the total mass (m1) was accurately recorded. The bottles were then placed in a desiccator containing desiccant for a total duration of t = 8 hours. For both the test and control groups, the weighing bottles were removed from the desiccator at 2, 4, 6, and 8 hours; the stoppers were secured, and the weight (mt) was accurately recorded.

Moisturizing performance was evaluated based on the percentage of weight loss due to drying.

Test Conclusion: Under the specified test conditions, the results showed that the percentage of weight loss for the test sample group at 2, 4, 6, and 8 hours was lower than that of the negative control group. This indicates that the “Bernach Recombinant Collagen Anti-Wrinkle Repair Mask” possesses moisturizing efficacy.

Repair

Study Protocol: A total of 33 healthy Asian (Chinese) male and female subjects were recruited for the test; all 33 were included in the valid analysis (100% validity rate). Instrumental measurements were used to compare changes in skin parameters before and after application of the test sample, thereby evaluating the repair efficacy of the submitted product.

Study Results and Conclusions:

Correlation between test parameters and efficacy claims

Repair: Helps maintain the treated area in a normal state.

Skin barrier function can be characterized by Transepidermal Water Loss (TEWL) values, Erythema Index (EI) values, and stratum corneum hydration levels. Therefore, the repair efficacy of a sample can be evaluated by measuring improvements in these parameters—specifically TEWL, EI, and stratum corneum hydration—in subjects before and after product application.

A lower TEWL value indicates reduced transepidermal water loss; alternatively, a lower EI value combined with higher stratum corneum hydration indicates superior skin barrier function, thereby demonstrating the sample‘s repair efficacy.